Microbiomics Made Simple

MIQ Score Portal

A pipeline to compare the observed and expected values from microbiome standards.

Standard

Select Sequencing Method

Choose the sequencing method to be run on your sample. Note: The current version of the MIQ Score pipeline only supports pair-end Illumina short-read data.

Parameters

Adjust values for generating your MIQ Score report.
Please enter a value between 0 and 30.
Please enter a value between 0 and 30.
Please enter a value between 0 and 600.
Example parameter values for common 16S library preparation processes.
Target Region Library Preparation Methods Forward Primer Length(bp) Reverse Primer Length(bp) Maximum Amplicon Length(bp)
V3-V4 Quick 16S Plus Kit(V3-V4), Illumina 16S Library Preparation Protocol 17 24 510
V4 Quick 16S Plus Kit(V4), Earth Microbiome Project 16S Protocol 0 0 280
V1-V3 Quick 16S Plus Kit(V1-V3) 19 20 540
V1-V2 Quick 16S Plus Kit(V1-V2) 17 20 450
  • If your library kit doesn’t require primer trimming, please enter 0 for both the forward and backward primer length.

Upload Files

Note: If your FASTQ files exceed the max file size, please consider subsetting your files before submitting. Paired FASTQ files exceed the allowed upload size.
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User Info

Your report will be sent to the email address entered below. Note: In case you don’t receive the report email after a while, please check you junk box.
Please enter a valid email.

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